Description:
Recombinant full-length Borrelia miyamotoi GlpQ protein produced in E. coli cells. Protein contains a C-terminal 10x His-tag. The protein was purified using immobilised metal affinity chromatography and ion exchange chromatography.
Buffer :
300 mM NaCl, 50 mM Tris-HCl, pH 8.5
Concentration:
1.72 mg/ml (according to BCA assay using BSA standards in 25 mM Tris-HCl, pH 7.6, 150 mM NaCl)
Purity:
> 90% by SDS-PAGE. GlpQ with a C-terminal linker and His-tag is 41kD
Volume:
58 μl (+3% overfill)
Freezing:
Freeze on arrival, but avoid multiple freeze/thaw cycles