Monkey FGF19 (Fibroblast Growth Factor 19) ELISA Kit

Product Type:

ELISA Kit

ELISA Type:

Sandwich

Assay Principle:

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey FGF19. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey FGF19 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey FGF19, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey FGF19. The concentration of Monkey FGF19 in samples can be calculated by comparing the OD of the samples to the standard curve.

Reactivity:

Monkey

Specificity/Target:

This kit recognizes monkey FGF19 in samples. No significant cross-reactivity or interference between Monkey FGF19 and analogues was observed.

Detection Range:

7.81-500 pg/ml

Sensitivity:

4.69 pg/ml

Assay Time:

4.5h

Sample Type:

Serum, plasma and other biological fluids

Detection Method:

Colormetric