Mouse Adiponectin ELISA Assay

Product Overview:

The mouse adiponectin ELISA assay kit measures adiponectin in mouse serum and plasma samples.

Assay Principle:

The mouse adiponectin ELISA assay kit is a so-called sandwich-assay using two specific and high affinity antibodies. The adiponectin in the samples binds to the first antibody coated on the microtiter plate. In the following step the second specific anti-adiponectin-antibody binds in turn to the immobilised adiponectin. The second antibody is biotinylated and will be applied in a mixture with a streptavidin-peroxidase-enzyme conjugate. In the closing substrate reaction the turn of the colour will be catalysed quantitatively depending on the Adiponectin-level of the samples.

Sensitivity:

0.008 ng/ml

Dynamic Range:

0.025 – 1 ng/ml

Incubation Time:

2.5 hours

Sample Type:

Plasma, serum

Sample Size:

10 µl

Specimen Collection and Handling:

Serum and plasma samples of mice and mouse cell culture medium can be used in this assay. Influence of Heparin (30 IE/mL), EDTA (6,8 mM) and NaCitrat (0,015 M) on the measurement of Adiponectin has been investigated be recovery experiments. PBS was enriched with recombinant mouse Adiponectin and the above mentioned substances. No significant influence on the recovery of adiponectin was detected.
Hemolytic reactions have to be avoided. The blood has to be allowed to clot and after complete clotting, serum is separated by centrifugation.