Mouse Alpha2-AP Active ELISA Assay

Product Overview:

The mouse alpha2-AP active ELISA assay is for the quantitative determination of active alpha 2-Antiplasmin (alpha2-AP) in mouse plasma by a microplate enzyme immunoassay (ELISA).

Product Description:

This Mouse Alpha-2-antiplasmin (Alpha2-AP) Active ELISA Assay kit is for the quantitative determination of active a-2-antiplasmin in mouse plasma. Alpha 2 antiplasmin (Alpha2-AP) is the major circulating inhibitor of plasmin, and it has a role in the regulation of intravascular fibrinolysis. Decreased levels of Alpha2 antiplasmin may play an important role in the increased capacity of the fibrinolytic function and may be beneficial in the treatment of thrombotic diseases, acute pulmonary embolism, and hepatic repair.

Sensitivity:

0.025 ng/ml

Dynamic Range:

0.025 – 10 ng/ml

Incubation Time:

1.5 hours

Sample Type:

Plasma

Sample Size:

100 µl

Specimen Collection and Handling:

Collect 9 volumes of blood in 1 volume of 0.1 M trisodium citrate or acidified citrate. Immediately after collection of blood, samples must be centrifuged at 3000 x g for 15 minutes. The plasma should be transferred to a clean plastic tube and must be stored on ice prior to analysis. The samples are stable on ice for up to 6 hours or freeze at –20°C or colder for extended storage. It is highly suggested to dilute unknowns 1:1000 in 3% BSA Blocking Buffer because of the high level of alpha-2-antiplasmin in normal mouse plasma and serum. The assay measures active alpha-2-antiplasmin in the 0 – 10 µg/ml range.