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Mouse anti Bovine CD14:RPE
Products Name
Mouse anti Bovine CD14:RPE
Cat.No.
SCSA0007
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Overview
Description:
Mouse anti bovine CD14, clone CC-G33 recognizes bovine CD14.
CD14 is a GPI-anchored membrane glycoprotein and monocyte/macrophage differentiation antigen, belonging to the lipopolysaccharide receptor family, also expressed weakly on microglia and Langerhans cells. CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation and the release of cytokines and the inflammatory response, and has been shown to upregulate the cell surface expression of adhesion molecules.
CD14 is a GPI-anchored membrane glycoprotein and monocyte/macrophage differentiation antigen, belonging to the lipopolysaccharide receptor family, also expressed weakly on microglia and Langerhans cells. CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation and the release of cytokines and the inflammatory response, and has been shown to upregulate the cell surface expression of adhesion molecules.
Product Form:
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized.
Reconstitution:
Reconstitute with 1.0 ml distilled water.
Preparation:
Purified IgG prepared by affinity chromatography on protein A.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide (NaN3).
1% Bovine serum albumin.
5% Sucrose
1% Bovine serum albumin.
5% Sucrose
Immunogen:
Partially purified polypeptides isolated from bovine leucocyte cell surface membrane.
Fusion Partners:
Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cell line.
Excitation Max (nm):
RPE 488 nm laser: 496.
RPE 561nm laser: 546.
RPE 561nm laser: 546.
Emission Max (nm):
RPE 488 nm laser: 578.
RPE 561nm laser: 578.
RPE 561nm laser: 578.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
Store at +4°C.
Note:
DO NOT FREEZE.
Should this product contain a precipitate we recommend microcentrifugation before use.
Should this product contain a precipitate we recommend microcentrifugation before use.
Product Type:
Monoclonal antibody
Clone:
CC-G33
Isotype:
IgG1
Specificity:
CD14
Species Cross-Reactivity:
Sheep, human
Target:
Bovine