Mouse anti Bovine CD172a

Description:

Mouse anti bovine CD172a, clone DH59B, recognizes bovine CD172a also known as SIRP alpha or MyD-1 antigen. CD172a is a transmembrane signal regulatory protein expressed primarily by macrophages, monocytes, dendritic cells, granulocytes, myeloid progenitors, hematopoietic stem cells, and neurons. The extracellular region of SIRP family consists of three immunoglobulin superfamily (IgSF) domains; two IgC and one IgV domain.

The IgV domain of CD172a binds to CD47. The binding domain of CD172a is analogous to that of immunoglobulins and T cell receptors and is involved in myeloid cell activation. However, signaling via CD172a is mainly inhibitory to cell function and phagocytosis. CD172a expressing cells are stimulated in Mycobacterium tuberculosis infection, influencing migration of dendritic cells and macrophages, phagocytosis, and granuloma formation.

Product Form:

Concentrated tissue culture supernatant - liquid.

Preparation:

Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter.

Buffer :

Serum free tissue culture medium containing proprietary protein free supplement.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).

Immunogen:

Cells from multiple species with the final screening of the fusion on cells from dog.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry, immunohistology - frozen.

Storage:

Store at +4°C.

Note:

DO NOT FREEZE.
Should this product contain a precipitate we recommend microcentrifugation before use.