Description:
Mouse anti bovine CD172a antibody, clone CC149 recognizes bovine CD172a, also known as MyD-1 antigen and SIRPA.
CD172a is a ~55 kDa single pass type 1 membrane protein belonging to the family of signal regulatory proteins (SIRP). CD172a has been identified as the receptor for CD47. Bovine CD172a is strongly expressed by splenic macrophages, monocytes and a subset of afferent lymph veiled cells (ALVC) and by dendritic cells in the skin.
Product Form:
Purified IgG conjugated to R. Phycoerythrin (RPE) -Cy5 - lyophilized.
Reconstitution:
Reconstitute with 1.0ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial.
Preparation:
Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide (NaN3).
1% Bovine serum albumin.
5% Sucrose
Immunogen:
Bovine afferent veiled dendritic cells.
Excitation Max (nm):
RPE-Cy5 488nm laser: 496.
Emission Max (nm):
RPE-Cy5 488nm laser: 667.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Note:
DO NOT FREEZE.
Should this product contain a precipitate we recommend microcentrifugation before use.
Product Type:
Monoclonal antibody