Mouse anti Bovine CD18

Description:

Mouse anti bovine CD18, clone BAQ30A, recognizes bovine CD18 also known as ITGB2 or integrin beta-2. CD18 is a receptor for ICAM1, ICAM2, ICAM3, and ICAM4. It recognizes the G-P-R motif in the fibrinogen alpha chain and is involved in leukocyte adhesion as well as transmigration of T cells and neutrophils.

Different from other organisms, the signal peptide of ruminant CD18, the beta subunit, is not cleaved and remains intact on the mature CD18 molecule, leukotoxin from Pasteurella haemolytica binds to the intact signal peptide on ruminant leukocytes causing acute inflammation and lung damage (Shanthalingam et al. 2016). Holstein cattle harbor a mutation in CD18 causing bovine leukocyte adhesion deficiency (BLAD) syndrome with decreased expression of beta 2 integrins and lowered susceptibility to cytolysis in Pasteurella infections.

Product Form:

Concentrated tissue culture supernatant - liquid.

Preparation:

Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter.

Buffer :

Serum free tissue culture medium containing proprietary protein free supplement.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).

Immunogen:

Cells from multiple species.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry, functional assays.

Storage:

Store at +4°C.

Note:

DO NOT FREEZE.
Should this product contain a precipitate we recommend microcentrifugation before use.