Mouse anti Bovine CD205:IL-A114, FITC
Products Name
Mouse anti Bovine CD205:IL-A114, FITC
Cat.No.
BCMA0023
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Overview
Description:
Mouse anti bovine CD205 antibody, clone IL-A114 recognizes bovine CD205 (also known as DEC205) and originally described as BoWC6. CD205 is a cell surface antigen with an approximate molecular weight of ~210-220 kDa.
CD205 is expressed on afferent lymph dendritic cells (veiled cells), B cells, a subset of T cells and by cortical and medullary thymocytes.
CD205 is expressed on afferent lymph dendritic cells (veiled cells), B cells, a subset of T cells and by cortical and medullary thymocytes.
Product Type:
Monoclonal antibody
Clone:
IL-A114
Isotype:
IgG1
Specificity:
CD205
Species Cross-Reactivity:
Sheep
Target:
Bovine
Product Form:
Purified IgG conjugated to fluorescein isothiocyanate isomer 1 (FITC) - liquid.
Preparation:
Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide (NaN3).
1% Bovine serum albumin.
1% Bovine serum albumin.
Approx. Protein Concentrations:
IgG concentration 0.1 mg/ml.
Fusion Partners:
Spleen cells from immunized BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
Excitation Max (nm):
FITC: 490.
Emission Max (nm):
FITC: 525.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
