Mouse anti Bovine CD21:FITC

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Mouse anti Bovine CD21:FITC
Products Name
Mouse anti Bovine CD21:FITC
Cat.No.
BCMA0029
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Overview

Description:
Mouse anti bovine CD21 monoclonal antibody, clone CC51 recognizes the bovine homologue of the human CD21 cell surface antigen, a 145 kDa single pass type I membrane glycoprotein containing multiple sushi domains. CD21 is also known as complement receptor type 2 (CR2). In cattle CD21 expression is restricted to B-cells. CD21 may be expressed on B-cells as either a long or a short form.
Product Form:
Purified IgG conjugated to fluorescein isothiocyanate isomer 1 (FITC) - liquid.
Preparation:
Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide (NaN3).
1% Bovine serum albumin.
Immunogen:
Bovine (Friesian cattle) mesenteric lymph node cells.
Approx. Protein Concentrations:
IgG concentration 0.1 mg/ml.
Fusion Partners:
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
Excitation Max (nm):
FITC: 490.
Emission Max (nm):
FITC: 525.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Product Type:
Monoclonal antibody
Clone:
CC51
Isotype:
IgG2b
Specificity:
CD21
Species Cross-Reactivity:
Pig, African buffalo
Target:
Bovine

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