Description: 
                    
                    
                        Mouse anti bovine CD21 monoclonal antibody, clone CC51 recognizes the bovine homologue of the human CD21 cell surface antigen, a 145 kDa single pass type I membrane glycoprotein containing multiple sushi domains. CD21 is also known as complement receptor type 2 (CR2). In cattle CD21 expression is restricted to B-cells. CD21 may be expressed on B-cells as either a long or a short form.                    
                 
                                                                                    
                    
                        Product Form: 
                    
                    
                        Purified IgG conjugated to RPE - liquid.                    
                 
                                    
                    
                        Reconstitution: 
                    
                    
                        Reconstitute with 1.0 ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial.                     
                 
                                    
                    
                        Preparation: 
                    
                    
                        Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.                    
                 
                                    
                    
                        Buffer : 
                    
                    
                        Phosphate buffered saline.                    
                 
                                    
                    
                        Preservative Stabilisers: 
                    
                    
                        0.09% Sodium azide (NaN3). 
1% Bovine serum albumin.
5% Sucrose.                    
                 
                                    
                    
                        Immunogen: 
                    
                    
                        Bovine (Friesian cattle) mesenteric lymph node cells.                    
                 
                                    
                    
                        Fusion Partners: 
                    
                    
                        Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.                    
                 
                                    
                    
                        Excitation Max (nm): 
                    
                    
                        RPE 488 nm laser: 496.                    
                 
                                    
                    
                        Emission Max (nm): 
                    
                    
                        RPE 488 nm laser: 578.                    
                 
                                    
                    
                        Regulatory: 
                    
                    
                        For research purposes only.                    
                 
                                    
                    
                        Guarantee: 
                    
                    
                        12 months from date of despatch.                    
                 
                                    
                    
                        Application: 
                    
                    
                        Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.                    
                 
                                    
                                    
                    
                        Note: 
                    
                    
                        DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.                    
                 
                            
                                                                                                                                            
                        
                            Product Type: 
                        
                        
                            Monoclonal antibody                        
                     
                                                
                                                
                                                
                                                
                        
                            Species Cross-Reactivity: 
                        
                        
                            Pig, African buffalo