Description:
Mouse anti bovine CD21 monoclonal antibody, clone CC21 recognizes the bovine CD21 cell surface antigen, a ~145 kDa single pass type I membrane glycoprotein containing multiple sushi domains. CD21 is also known as complement receptor type 2. In cattle CD21 expression is restricted to B lymphocytes. CD21 may be expressed on B cells as either a long or a short form.
Mouse anti bovine CD21, clone CC21 has been used to demonstrate the co-expression of CD21 with PrPc on B cells of scrapie infected sheep.
Product Form:
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized.
Reconstitution:
Reconstitute with 1ml distilled water.
Preparation:
Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide.
1% Bovine serum albumin.
5% Sucrose.
Fusion Partners:
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
Excitation Max (nm):
RPE 488 nm laser: 496.
Emission Max (nm):
RPE 488 nm laser: 578.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
Prior to reconstitution Store at +4°C.
After reconstitution Store at +4°C.
Note:
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Product Type:
Monoclonal antibody
Species Cross-Reactivity:
Goat, sheep