Mouse anti Bovine CD21:RPE

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Mouse anti Bovine CD21:RPE
Products Name
Mouse anti Bovine CD21:RPE
Cat.No.
BCMA0031
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Overview

Description:
Mouse anti bovine CD21 monoclonal antibody, clone CC51 recognizes the bovine homologue of the human CD21 cell surface antigen, a 145 kDa single pass type I membrane glycoprotein containing multiple sushi domains. CD21 is also known as complement receptor type 2 (CR2). In cattle CD21 expression is restricted to B-cells. CD21 may be expressed on B-cells as either a long or a short form.
Product Type:
Monoclonal antibody
Clone:
CC51
Isotype:
IgG2b
Specificity:
CD21
Species Cross-Reactivity:
Pig, African buffalo
Target:
Bovine
Product Form:
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized.
Reconstitution:
Reconstitute with 1.0 ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial.
Preparation:
Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide (NaN3).
1% Bovine serum albumin.
5% Sucrose.
Immunogen:
Bovine (Friesian cattle) mesenteric lymph node cells.
Fusion Partners:
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
Excitation Max (nm):
RPE 488 nm laser: 496.
Emission Max (nm):
RPE 488 nm laser: 578.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
Store at +4°C.
Note:
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.

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