Description:
Mouse anti bovine CD25 antibody, clone IL-A111 recognizes the bovine CD25 cell surface antigen, a ~55 kDa glycoprotein also known as Interleukin-2 receptor alpha chain. Bovine CD25 is expressed by activated T cells.
Mouse anti bovine CD25 antibody, clone IL-A111 is reported to block the IL-2 driven proliferation of Con A-induced blast cells/ bovine lymphocytes.
Product Form:
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized.
Reconstitution:
Reconstitute with 1 ml distilled water.
Preparation:
Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide.
1% Bovine serum albumin.
5% Sucrose.
Fusion Partners:
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
Excitation Max (nm):
RPE 488 nm laser: 496.
Emission Max (nm):
RPE 488 nm laser: 578.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
Prior to reconstitution Store at +4°C.
Following reconstitution Store at +4°C.
Note:
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Product Type:
Monoclonal antibody
Species Cross-Reactivity:
Sheep