Overview

Description:

Mouse anti bovine CD28, clone CC219, recognizes the bovine homologue of human CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). CD28 is involved in increased production of IL-4 and IL-10 in T cells.

Mouse anti bovine CD28, clone CC219 is able to stimulate bovine T cells in combination with anti-bovine CD3. For functional assays it is recommended to use the low endotoxin and preservative free format of this antibody.

Product Form:

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized.

Reconstitution:

Reconstitute with 1.0 ml distilled water.

Preparation:

Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).
1% Bovine serum albumin.
5% Sucrose.

Immunogen:

Plasmid DNA encoding the bovine CD28 gene.

Fusion Partners:

Spleen cells from immunized Balb/c mice were fused with cells of the SP/2/0 myeloma cell line.

Excitation Max (nm):

RPE 488 nm laser: 496.

Emission Max (nm):

RPE 488 nm laser: 578.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry: use 10 µl of the suggested working dilution to label 10⁶ cells in 100 µl.

Storage:

Prior to reconstitution store at +4°C.
Following reconstitution store at +4°C.

Note:

DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Product Type:

Monoclonal antibody

Clone:

CC219

Isotype:

IgG1

Specificity:

CD28

Target:

Bovine

Mouse anti Bovine CD28:RPE

Overview

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