Mouse anti Bovine CD40:RPE
Products Name
Mouse anti Bovine CD40:RPE
Cat.No.
BCMA0057
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Overview
Description:
Mouse anti bovine CD40 antibody, clone IL-A156 recognizes the bovine Tumor necrosis factor receptor superfamily member 5, also known as CD40 or B-cell surface antigen CD40. CD40 is a 260 amino acid single pass type 1 transmembrane glycoprotein with a predicted molecular mass of 31 kDa. Bovine CD40 also has an N terminal signal peptide of 20 amino acids. Bovine CD40 is expressed by B lymphocytes and a subset of T lymphocytes.
CD40 plays an important role in the differentiation of B lymphocytes into effector cells, and is also involved in interactions between T and B lymphocytes.
CD40 plays an important role in the differentiation of B lymphocytes into effector cells, and is also involved in interactions between T and B lymphocytes.
Product Form:
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized.
Reconstitution:
Reconstitute with 1.0 ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial.
Preparation:
Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide (NaN3).
1% Bovine serum albumin.
5% Sucrose.
1% Bovine serum albumin.
5% Sucrose.
Fusion Partners:
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line
Excitation Max (nm):
RPE 488 nm laser: 496.
Emission Max (nm):
RPE 488 nm laser: 578.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
Prior to reconstitution store at +4°C.
Following reconstitution store at +4°C.
Following reconstitution store at +4°C.
Note:
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Product Type:
Monoclonal antibody
Clone:
IL-A156
Isotype:
IgG1
Specificity:
CD40
Species Cross-Reactivity:
Sheep
Target:
Bovine
