Description:
Mouse anti bovine CD45RB, clone CC76, recognizes the RB isoform of the bovine leucocyte common antigen expressed on subpopulations of CD4+ and CD8+ T cells. Expression levels of CD45RB are used as a marker for differentiating naïve and memory T lymphocytes. The antigen recognized by clone CC76 is also expressed on B cells but is absent from both macrophages and granulocytes.
Product Form:
Purified IgG - liquid.
Preparation:
Purified IgG prepared by affinity chromatography on protein A.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide (NaN3).
Immunogen:
Bovine thymocytes.
Approx. Protein Concentrations:
IgG concentration 1.0 mg/ml.
Fusion Partners:
Spleen cells from immunised BALB/c micewere fused with cells of the NS1 myeloma cell line.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Product Type:
Monoclonal antibody