Mouse anti Bovine CD45RO

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Mouse anti Bovine CD45RO
Products Name
Mouse anti Bovine CD45RO
Cat.No.
MACD0038
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Overview

Description:
Mouse anti bovine CD45RO, clone IL-A116 recognizes the bovine homologue of the human CD45RO cell surface antigen.
CD45, also known as Leucocyte Common Antigen or LCA, occurs in a number of isoforms, clone IL-A116 is specific for the low molecular weight isoform termed CD45RO, the isoform associated with expression on memory T-cells. Bovine CD45RO is expressed by monocytes, granulocytes and subsets of thymocytes, CD4+ T cells and CD8+ T cells. CD45RO+ CD8+ T cells increase from approximately 5% in neonatal calves to approximately 35% in adult cattle over the age of 5 years. Mouse anti Bovine CD45RO, clone IL-A116 immunoprecipitates a molecule of ~180 kDa, analogus to the molecular weight of human and mouse CD45RO.
Product Form:
Purified IgG - liquid.
Preparation:
Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide (NaN3).
Carrier Free:
Yes.
Immunogen:
Bovine peripheral blood monouclear cells.
Approx. Protein Concentrations:
IgG concentration 1.0 mg/ml.
Fusion Partners:
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry, immunoprecipitation.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Product Type:
Monoclonal antibody
Clone:
IL-A116
Isotype:
IgG3
Specificity:
CD45RO
Species Cross-Reactivity:
Goat
Target:
Bovine

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