Mouse anti Bovine CD4/CC30

Description:

Mouse anti bovine CD4 antibody, clone CC30 recognizes a ~50 kDa transmembrane molecule considered to be the bovine homologue of human CD4. The phenotype, tissue distribution and function of T-cells expressing the bovine CD4 antigen are similar to those in other species. However, expression on macrophages has not yet been detected.
Mouse anti bovine CD4, clone CC30 has successfully been used for immunohistochemical localization of CD4 on paraffin embedded material using zinc salt fixation. Additionally, clone CC30 has been reported as being suitable for use on formal dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).

Carrier Free:

Yes.

Immunogen:

Bovine thymocytes.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Fusion Partners:

Spleen cells from an immunised mouse were fused with cells of the mouse NS1 myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry, immunohistology - frozen, immunohistology - paraffin, immunoprecipitation.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.