Mouse anti Bovine IgA:HRP

Description:

Mouse anti bovine IgA, clone IL-A71, is specific for bovine immunoglobulin A (IgA).

IgA is the predominant surface secreted immunoglobulin from the intestinal, urogenital and respiratory tracts. IgA functions in a protective capacity, primarily, by preventing viruses and bacteria from adhering to epithelial tissues. High concentrations of IgA are found in saliva, colostrum, milk, tear duct fluid, urine and nasal fluid.

Clone IL-A71 is reported to immunoprecipitate bands of approximately 62kDa and 75kDa, which is consistent with the heavy chain and the secretory component of IgA.

Product Form:

Purified IgG conjugated to HRP - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.01% Thiomersal.

Immunogen:

Purified bovine Immunoglobulin.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Fusion Partners:

Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

ELISA.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.