Description:
Mouse anti bovine IgM, clone IL-A30 recognizes bovine IgM, expressed by bovine B-cells. Clone IL-A30 reacts with both serum IgM and cell surface IgM expressed by B lymphocytes. Mouse anti bovine IgM, clone IL-A30 recognizes a monomorphic epitope within bovine IgM.
The percentage of IgM lymphocytes may vary widely ,ranging between 4 - 30%, within populations of healthy cattle, however the percentage of positive cells remains relatively constant within individual animals.
Product Form:
Purified IgG - liquid.
Preparation:
Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide (NaN3).
Immunogen:
Bovine peripheral blood mononuclear cells depleted of T lymphocytes.
Approx. Protein Concentrations:
IgG concentration 1.0 mg/ml.
Fusion Partners:
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
ELISA, flow cytometry, immunoprecipitation,radioimmunoassays.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Product Type:
Monoclonal antibody