Mouse anti Bovine Interferon Gamma

Description:

Mouse anti bovine interferon gamma antibody, clone 5D10, recognises IFNγ, a 19.3 kDa protein belonging to the type II interferon family .

Bovine IFNγ is produced by activated lymphocytes, has anti-viral and immunoregulatory properties and is an activator of macrophages. Clone 5D10 is one of a range of bovine IFNγ reagents available from BioVenic which include the monoclonal antibody clones CC330, CC302 and 7B6 along with recombinant bovine IFNγ and bovine IFNγ ELISA kits.

Mouse anti bovine interferon gamma antibody, clone 5D10 is suitable for ELISA and ELISpot, for intracellular identification of bovine IFNγ and may be used in conjuction with clone CC302 (clone CC302 is available in a range of direct conjugates) as a detection reagent.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).

Carrier Free:

Yes.

Immunogen:

Purified recombinant bovine IFN gamma expressed in E.coli.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Fusion Partners:

Spleen cells from immunised Balb/c mice were fused with cells of the Sp20/Ag-14 myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

ELISA, ELISpot.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.