Mouse anti Bovine Interferon Gamma:FITC

Description:

Mouse anti bovine IFNγ antibody, clone CC302 recognizes bovine interferon-gamma, a 143 amino acid cytokine with potent activating, antiviral and anti proliferative properties, produced as a pro-peptide with an additional 23 amino acid N-terminal signal peptide sequence having a molecular weight of ~20 kDa. IFNγ is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection.

Mouse anti bovine γ interferon antibody, clone CC302 has been demonstrated to be reactive to a number of mammalian species including human, sheep, dog, pig, goat and mink. Mouse anti Bovine IFNγ antibody, clone CC302 has been used successfully for the evaluation of γ interferon levels in the sera of calves naturally infected with M. avium. subsp paratuberculosis as a detection reagent using an ELISA.

Product Type:

Monoclonal antibody

Clone:

CC302

Isotype:

IgG1

Specificity:

IFN Gamma

Species Cross-Reactivity:

Human, pig, dog, horse, sheep, goat, rabbit

Target:

Bovine

Product Form:

Purified IgG conjugated to FITC - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide.
1% Bovine serum albumin.

Approx. Protein Concentrations:

IgG concentration 0.1 mg/ml.

Fusion Partners:

Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Excitation Max (nm):

FITC: 490.

Emission Max (nm):

FITC: 525.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry: use 10 µl of the suggested working dilution to label 10⁶ cells in 100 µl.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.