Mouse anti Bovine Interferon Gamma:RPE

Description:

Mouse anti bovine IFNγ antibody, clone CC302 recognizes bovine interferon-gamma, a 143 amino acid cytokine with potent activating, antiviral and anti proliferative properties, produced as a pro-peptide with an additional 23 amino acid N-terminal signal peptide sequence having a molecular weight of ~20 kDa. IFNγ is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection.

Mouse anti bovine γ interferon antibody, clone CC302 has been demonstrated to be reactive to a number of mammalian species including human, sheep, dog, pig, goat and mink. Mouse anti Bovine IFNγ antibody, clone CC302 has been used successfully for the evaluation of γ interferon levels in the sera of calves naturally infected with M. avium. subsp paratuberculosis as a detection reagent using an ELISA.

Product Form:

Purified IgG conjugated to RPE - liquid.

Reconstitution:

Reconstitute with 1 ml distilled water.

Preparation:

Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide.
1% Bovine serum albumin.
5% Sucrose.

Fusion Partners:

Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Excitation Max (nm):

RPE 488 nm laser: 496.

Emission Max (nm):

RPE 488 nm laser: 578.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry: use 10 µl of the suggested working dilution to label 10⁶ cells in 100 µl.

Storage:

Store at +4°C DO NOT FREEZE.

Note:

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend micro-centrifugation before use.