Mouse anti Bovine Interleukin-4

Description:

Mouse anti bovine interleukin-4, clone CC313 is specific for bovine interleukin 4 (IL-4), also known as B-cell stimulatory factor 1. Bovine interleukin-4 is a 15 kDa, 135 amino acid peptide which is processed to a mature secreted form with the cleavage of a 24 amino acid, N-terminal signal peptide resulting in a 12.6 kDa active cytokine, the apparent MW may be altered by glycosylation.

IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses.

Mouse anti bovine interleukin-4, clone CC313 has been used successfully as a coating antibody in a sandwich ELISA with biotinylated mouse anti bovine IL-4 antibody, clone CC314 as a detection reagent both with bovine and ovine samples. Clones CC313 and CC314 have been used in the reverse configuration for the measurement of interleukin-4 in caprine samples.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide.

Carrier Free:

Yes.

Immunogen:

Recombinant bovine IL-4.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Fusion Partners:

Spleen cells form immunized BALB/c mice were fused with cells of the SP2/0 myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

ELISA.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.