Description:
Mouse anti bovine MHC Class I Monomorphic antibody, clone IL-A88 recognizes a monomorphic determinant within the heavy chain of bovine MHC Class I.
Bovine MHC class I molecules are expressed at varying levels on most nucleated cells, with exception of neural cells. This antibody immunoprecipitates a band of approximately 45 kDa under reducing conditions.
Product Form:
Purified IgG - liquid.
Preparation:
Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide (NaN3).
Immunogen:
Bovine peripheral blood mononuclear cells.
Approx. Protein Concentrations:
IgG concentration 1.0 mg/ml.
Fusion Partners:
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry, Western blotting.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Product Type:
Monoclonal antibody
Specificity:
MHC Class I Monomorphic