Mouse anti Bovine MHC Class I Monomorphic:FITC
Products Name
Mouse anti Bovine MHC Class I Monomorphic:FITC
Cat.No.
BCMA0079
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Overview
Description:
Mouse anti bovine MHC Class I Monomorphic antibody, clone IL-A88 recognizes a monomorphic determinant within the heavy chain of bovine MHC Class I.
Bovine MHC class I molecules are expressed at varying levels on most nucleated cells, with exception of neural cells. This antibody immunoprecipitates a band of approximately 45 kDa under reducing conditions.
Bovine MHC class I molecules are expressed at varying levels on most nucleated cells, with exception of neural cells. This antibody immunoprecipitates a band of approximately 45 kDa under reducing conditions.
Product Type:
Monoclonal antibody
Clone:
IL-A88
Isotype:
IgG2a
Specificity:
MHC Class I Monomorphic
Target:
Bovine
Product Form:
Purified IgG conjugated to FITC - liquid.
Preparation:
Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide (NaN3).
1% Bovine serum albumin.
1% Bovine serum albumin.
Immunogen:
Bovine peripheral blood mononuclear cells.
Approx. Protein Concentrations:
IgG concentration 0.1 mg/ml.
Fusion Partners:
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
Excitation Max (nm):
FITC: 490.
Emission Max (nm):
FITC: 525.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
