Mouse anti Bovine WC1/CC15:FITC
Products Name
Mouse anti Bovine WC1/CC15:FITC
Cat.No.
BCMA0091
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Overview
Description:
Mouse anti bovine WC1 antibody, clone CC15 recognizes bovine WC1.
WC1 is a ~215/300 kDa antigen expressed on the majority of gamma/delta T lymphocytes. These cells also express low levels of CD5, but are negative for other B and T cell markers. The WC1 antigen is reported to be involved in a number of processes, including activation of gamma/delta T cells and the development of a Th1-biased acquired immune response.
Mouse anti bovine WC1 antibody, clone CC15 is routinely tested in flow cytometry on bovine peripheral blood lymphocytes.
WC1 is a ~215/300 kDa antigen expressed on the majority of gamma/delta T lymphocytes. These cells also express low levels of CD5, but are negative for other B and T cell markers. The WC1 antigen is reported to be involved in a number of processes, including activation of gamma/delta T cells and the development of a Th1-biased acquired immune response.
Mouse anti bovine WC1 antibody, clone CC15 is routinely tested in flow cytometry on bovine peripheral blood lymphocytes.
Product Type:
Monoclonal antibody
Clone:
CC15
Isotype:
IgG2a
Specificity:
WC1
Species Cross-Reactivity:
Goat, sheep
Target:
Bovine
Product Form:
Purified IgG conjugated to FITC - liquid.
Preparation:
Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide.
1% Bovine serum albumin.
1% Bovine serum albumin.
Immunogen:
Bovine lymphocytes
Approx. Protein Concentrations:
IgG concentration 0.1 mg/ml.
Fusion Partners:
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Excitation Max (nm):
FITC: 490.
Emission Max (nm):
FITC: 525.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
