Mouse anti Canine CD21:RPE

Description:

Mouse anti canine CD21 antibody, clone CA2.1D6 recognizes canine CD21, also known as Complement receptor type 2. CD21 is a cell surface antigen expressed by canine B lymphocytes.

The antigen recognized may be the canine homologue of human CD21, but this has not been fully confirmed.

Mouse anti canine CD21 antibody , clone CA2.1D6 also recognizes the CD21 antigen in Felids. Expression in cats is analogous to that seen in dogs with strong expression on lymphocytes, in a manner mutually exclusive with expression of CD4 or CD8. Mouse anti canine CD21 antibody, clone CA2.1D6 immunoprecipitates a ~145 kDa protein from feline lymphocytes, similar to the protein immunoprecipitated by the antibody from canine lymphocytes.

Product Form:

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized.

Reconstitution:

Reconstitute with 1 ml distilled water.

Preparation:

Purified IgG prepared by affinity chromatography on protein G.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide.
1% Bovine serum albumin.
5% Sucrose.

Excitation Max (nm):

RPE 488 nm laser: 496.

Emission Max (nm):

RPE 488 nm laser: 578.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry: use 10 µl of the suggested working dilution to label 10⁶ cells or 100 µl whole blood.

Storage:

Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.

Note:

DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.