Mouse anti Chicken CD268/BAFF-R

Description:

Mouse anti chicken CD268/BAFF-R, clone 2C4, recognizes the chicken homologue of human BAFF-R, known also as CD268 and TNFRSF 13C, a cell surface antigen expressed by avian B-cells.

Chicken BAFF-R (B cell activating factor receptor) differs from mammalian BAFF-R in that it is produced in B-cells and is not expressed by chicken T-cells. In addition to chicken, clone 2C4 cross reacts with several other commercially important avian species and as such is a specific marker of B-cells for research in domestic galliforms.

Mouse anti chicken CD268/BAFF-R, clone 2C4 is unique in that it can be used in formalin fixed paraffin embedded tissues and is therefore of particular use on sections from infected specimens.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

< 0.1% Sodium azide (NaN₃).

Immunogen:

Chicken BAFF-R transfected HEK293 cells.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry, immunohistology - frozen, immunohistology - paraffin.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.