Mouse anti Chicken CD3

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Mouse anti Chicken CD3
Products Name
Mouse anti Chicken CD3
Cat.No.
MAPC0012
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Overview

Description:
Mouse anti chicken CD3, clone CT-3 recognizes the chicken CD3 cell surface antigen expressed by T cells. The antibody also co-precipitates a ~90 kDa heterodimer from digitonin solubilised T cell lysates, which can be reduced to two polypeptides of ~40 kDa and ~50 kDa.

In the chicken, CD3 is expressed by approximately 30% of thymocytes, 70% of peripheral blood mononuclear cells, but <1% of cells in the bursa and bone marrow.
Product Form:
Purified IgG - liquid.
Preparation:
Purified IgG prepared from ascites.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
None present.
Immunogen:
Chicken thymocytes and Ig -ve peripheral blood mononuclear cells.
Approx. Protein Concentrations:
IgG concentration 0.5 mg/ml.
Fusion Partners:
Lymph node cells from immunised Balb/c mice were fused with cells from the mouse P3-X63Ag8.653 myeloma cell line.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry, immunohistology - frozen, immunoprecipitation.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Product Type:
Monoclonal antibody
Clone:
CT-3
Isotype:
IgG1
Specificity:
CD3
Target:
Chicken

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