Mouse anti Chicken CD45

Description:

Mouse anti chicken CD45 antibody, clone UM16-6 recognizes chicken CD45, also known as leucocyte common antigen. CD45 is a heavily-glycosylated transmembrane protein tyrosine phosphatase (PTPase) expressed by all nucleated cells of haematopoietic origin. Variation in the expression of a particular CD45 isoform, is regulated during the haematopoietic development of the different cell lineages.

CD45 is essential for antigen-induced signal transduction through the antigen receptor and as with other PTPase family members, acts in balance with protein tyrosine kinases, causing the dephosphorylation of negative regulatory tyrosine sites. De-phosphorylation by CD45, is required for the activation of the src-family kinases p56^lck and p59^fyn.

Chicken CD45 has an additional cysteine residue near the transmembrane region compared to human and shark CD45. The overall domain structure between mammalian and chicken CD45 appears to be conserved, but the sequence homology between the extracellular regions is very low.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by ion exchange chromatography from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).

Immunogen:

COS cells transfected with CD45 gene.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry, immunohistology - frozen, immunoprecipitation.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.