Mouse Anti-Chicken CD45 (LT40)

Description:

Chicken CD45 is a transmembrane glycoprotein expressed on all leukocytes. B lineage cells in the bursa of Fabricius express the 215 kDa isoform while T lineage cells bear the 190 kDa variant. These high molecular weight molecules have intrinsic phosphotyrosine phosphatase activity characteristic of mammalian CD45. Levels of CD45 expression detected by the monoclonal antibody LT40 in the avian thymus are heterogeneous with approximately 90% of thymocytes expressing fourfold higher levels of surface CD45 (CD45^hi) than do the remaining 10% (CD45^low) of thymocytes. The CD45^low population contains exclusively thymocytes with the CD3^-CD4^-CD8^low phenotype characteristic of the immediate precursors to the CD3^-CD4⁺CD8⁺ thymic population which are CD45^hi. This shift from low to high levels of surface CD45 expression is concomitant with the transition from CD4-CD8^low to CD4⁺CD8⁺ and before the expression of CD3. The ability of the monoclonal antibody LT40 to discriminate this CD45^low subpopulation provides a suitable marker for the CD3^-CD4^-CD8^low immediate precursors to the CD3^-CD4⁺CD8⁺ thymocytes.

Reactivity:

Chicken

Host:

Mouse

Immunogen:

Chicken bursal lymphocytes

Buffer :

Phosphate buffered saline containing < 0.1% sodium azide

Clonality:

Monoclonal

Concentration:

0.5 mg/mL

Volume:

0.2 mL

Recommended Storage:

2-8°C, Avoid exposure to light

Application:

Flow cytometry
Immunohistochemistry-frozen sections
Immunohistochemistry-paraffin sections
Immunohistochemistry-whole mount
Immunocytochemistry
Immunoprecipitation
Separation