Mouse anti Chicken CD45:RPE

Description:

Mouse anti chicken CD45 antibody, clone UM16-6 recognizes chicken CD45, also known as leucocyte common antigen. CD45 is a heavily-glycosylated transmembrane protein tyrosine phosphatase (PTPase) expressed by all nucleated cells of haematopoietic origin. Variation in the expression of a particular CD45 isoform, is regulated during the haematopoietic development of the different cell lineages.

CD45 is essential for antigen-induced signal transduction through the antigen receptor and as with other PTPase family members, acts in balance with protein tyrosine kinases, causing the dephosphorylation of negative regulatory tyrosine sites. De-phosphorylation by CD45, is required for the activation of the src-family kinases p56^lck and p59^fyn.

Chicken CD45 has an additional cysteine residue near the transmembrane region compared to human and shark CD45. The overall domain structure between mammalian and chicken CD45 appears to be conserved, but the sequence homology between the extracellular regions is very low.

Product Form:

Purified IgG conjugated to RPE - liquid.

Reconstitution:

Reconstitute with 1.0 ml distilled water.

Preparation:

Purified IgG prepared by affinity chromatography on protein G from ascites.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide.
1% Bovine serum albumin.

Immunogen:

COS cells transfected with CD45 gene.

Excitation Max (nm):

RPE 488 nm laser: 496.

Emission Max (nm):

RPE 488 nm laser: 578.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry: use 10 µl of the suggested working dilution to label 10⁶ cells in 100 µl.

Storage:

Prior to reconstitution Store at +4°C.
After reconstitution Store at +4°C.

Note:

DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.