Mouse Anti-Chicken CD8α-PE (CT-8)

Description:

In the chicken, the CD8 molecule is present in two forms - (i) a homodimer of two α chains and (ii) a heterodimer of an α chain and a β chain. While the vast majority of CD8⁺ cells in the thymus, spleen, and blood of adult chickens express both CD8α- and CD8β-chains, a relatively large proportion of the CD8⁺ TCRγδ cells in the spleens of embryos and young chicks express only the α-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8⁺ T cell populations present in mice are conserved but there is a population of TCRγδ CD8αβ cells in the chicken that is not found in rodents. Chicken CD8 is expressed on approximately 80% of thymocytes, 15% of blood mononuclear cells, and 50% of spleen cells but less than 1% of cells in the bursa and bone marrow. The monoclonal antibody CT-8 recognizes the CD8α chain and has been shown to react to a polymorphic determinant in turkey.

Reactivity:

Chicken, Turkey, Guinea Fowl, Pigeon

Host:

Mouse

Immunogen:

Chicken thymocytes and Ig-negative blood leukocytes

Conjugate:

PE (R-phycoerythrin)

Buffer :

Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer

Clonality:

Monoclonal

Concentration:

0.1 mg/mL

Volume:

1.0 mL

Recommended Storage:

2-8°C, Avoid exposure to light, Do not freeze

Application:

Flow cytometry
Immunohistochemistry-frozen sections
Immunohistochemistry-paraffin sections
Immunoprecipitation
Blocking