Mouse anti Chicken Interferon Alpha

Description:

Mouse anti chicken interferon alpha antibody, clone UMCh3-IFNa recognizes chicken Interferon alpha (chIFN-alpha).
The classification of mammalian cytokines with antiviral activity, is well documented as being either Type I Interferon (IFNI) or Type II Interferon (IFNII). This is not the case for avian IFNs however, even though IFN was first detected in the chicken.
There are similarities between chicken IFN and mammalian IFNI with conservation of cysteine residues and the existence of around ten closely related intron-less IFN alpha genes. Chicken IFN-alpha genes are strongly expressed following viral infection of monocyte-derived macrophage and embryo fibroblasts and that chicken IFN acts as a potent antiviral agent.
Mouse anti chicken interferon alpha antibody, clone UMCh3-IFNa does not cross react with IFN beta or IFN gamma and does not neutralize chIFN-alpha.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein G from ascites.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

< 0.1% Sodium azide (NaN₃).

Immunogen:

Recombinant chicken Interferon alpha.

Approx. Protein Concentrations:

IgG concentration 0.5 mg/ml.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

ELISA, western blotting.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.