Mouse Anti-Chicken TCRγδ-PE (TCR-1)

Description:

The monoclonal antibody TCR-1 precipitates a heterodimer of Mr 90 kDa (two bands of Mr 50 kDa and 40 kDa upon reduction) on chicken peripheral blood T cells. Deglycosylation of the heterodimer yields two polypeptides of Mr 35 kDa and 32 kDa. Unlike humans and mice in which γδ cells comprise a minor subset of T lymphocytes in the circulation, the chicken has a relatively large subset of γδ T cells. The frequency of TCRγδ cells is typically 20-25% of the total blood T cells but may reach approximately 50% in chickens of six- months of age. The majority of TCRγδ⁺ cells in the thymus and blood are CD4^-CD8^- although a small subset of them may express CD8 or CD4 coreceptors. However, when the TCRγδ⁺ cells migrate into the spleen and intestine most of them begin to express CD8. Although the biological function of γδ T cells is unclear, they are clearly capable of cytotoxic activity in vitro. The CD8⁺ γδ T cells may also be involved in the down-regulation of the immune response. They cannot, however, induce a graft-vs-host (GVH) reaction.

Clone:

TCR-1

Isotype:

Mouse (BALB/c) IgG1κ

Reactivity:

Chicken, Milk Snake

Host:

Mouse

Target:

TCRγδ

Specificity:

Chicken/Milk Snake TCRγδ

Immunogen:

Outbred chicken thymocytes and Ig-negative blood lymphocytes

Alternative Names:

T3/TCR complex, TCR gamma/delta

Conjugate:

PE (R-phycoerythrin)

Buffer :

Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer

Clonality:

Monoclonal

Concentration:

0.1 mg/mL

Volume:

1.0 mL

Recommended Storage:

2-8°C, Avoid exposure to light, Do not freeze

Application:

Flow cytometry
Immunohistochemistry-frozen sections
Immunohistochemistry-paraffin sections
Immunoprecipitation
Depletion