Mouse Anti-Chicken TCRαβ/Vβ2-FITC (TCR-3)

Description:

The monoclonal antibody TCR-3 precipitates a CD3-associated heterodimer of Mr 88 kDa (two bands of Mr 48 kDa and 40 kDa upon reduction) on chicken peripheral blood T cells. Deglycosylation of the heterodimer yields two polypeptides of Mr 34 kDa and 31 kDa. In the chicken, two distinct subpopulations of αβ T cells appear in the thymus subsequent to the appearance of γδ T cells. These subpopulations, originally denoted as TCR-2 and TCR-3, arise sequentially in the thymus during ontogeny and are now known to represent two distinct Vβ families, Vβ1 and Vβ2, respectively. The TCR-3 monoclonal antibody reacts with approximately 9% of thymocytes, 15-25% of blood mononuclear cells, and 13% of splenocytes young adult chickens. Two-color immunofluorescence has revealed that the TCR-3⁺ thymocytes include CD4⁺CD8^-, CD4-CD8⁺, CD4⁺CD8⁺, and CD4^-CD8^- subpopulations. The TCR-3⁺ thymocytes can be separated into two subsets. One subset is characterized by relatively low levels of expression of the TCR-3/CD3 complex and most of the cells in this subset are CD4⁺CD8⁺. Cells in the other subset express TCR-3/CD3 in higher density and are either CD4⁺CD8^- or CD4^-CD8⁺, corresponding to the more mature medullary subset of thymocytes.

Reactivity:

Chicken, Turkey

Host:

Mouse

Immunogen:

CD3⁺ TCR1^- TCR2^- Ia^-chicken blood mononuclear cells

Alternative Names:

T3/TCR complex, TCR alpha/beta

Conjugate:

FITC (Fluorescein)

Buffer :

Phosphate buffered saline containing < 0.1% sodium azide

Clonality:

Monoclonal

Concentration:

0.5 mg/mL

Volume:

1.0 mL

Recommended Storage:

2-8°C, Avoid exposure to light

Application:

Flow cytometry
Immunohistochemistry-frozen sections
Immunoprecipitation
Depletion