Mouse Anti-Chicken TCRαβ/Vβ2-PE (TCR-3)

Description:

The monoclonal antibody TCR-3 precipitates a CD3-associated heterodimer of Mr 88 kDa (two bands of Mr 48 kDa and 40 kDa upon reduction) on chicken peripheral blood T cells. Deglycosylation of the heterodimer yields two polypeptides of Mr 34 kDa and 31 kDa. In the chicken, two distinct subpopulations of αβ T cells appear in the thymus subsequent to the appearance of γδ T cells. These subpopulations, originally denoted as TCR-2 and TCR-3, arise sequentially in the thymus during ontogeny and are now known to represent two distinct Vβ families, Vβ1 and Vβ2, respectively. The TCR-3 monoclonal antibody reacts with approximately 9% of thymocytes, 15-25% of blood mononuclear cells, and 13% of splenocytes young adult chickens. Two-color immunofluorescence has revealed that the TCR-3 thymocytes include CD4CD8, CD4CD8, CD4CD8, and CD4CD8 subpopulations. The TCR-3 thymocytes can be separated into two subsets. One subset is characterized by relatively low levels of expression of the TCR-3/CD3 complex and most of the cells in this subset are CD4CD8. Cells in the other subset express TCR-3/CD3 in higher density and are either CD4CD8 or CD4CD8, corresponding to the more mature medullary subset of thymocytes.

Reactivity:

Chicken, Guinea Fowl, Peacock

Host:

Mouse

Immunogen:

CD3 TCR1 TCR2 Ia chicken blood mononuclear cells

Alternative Names:

T3/TCR complex, TCR alpha/beta

Conjugate:

PE (R-phycoerythrin)

Buffer :

Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer

Clonality:

Monoclonal

Concentration:

0.1 mg/mL

Volume:

1.0 mL

Recommended Storage:

2-8°C, Avoid exposure to light, Do not freeze

Application:

Flow cytometry
Immunohistochemistry-frozen sections
Immunoprecipitation