Mouse anti Dog CD18
Products Name
Mouse anti Dog CD18
Cat.No.
ARECSA0010
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Overview
Description:
Mouse anti dog CD18 antibody, clone CA1.4E9 recognizes the canine CD18 cell surface antigen, also known as the b2 integrin.
The tissue and cellular distribution of CD18 in canine tissue closely follows that observed for humans CD18 is expressed by virtually all leucocytes, but more strongly upon monocytes and granulocytes than on lymphocytes. The cross reactivity patterns of mouse anti canine CD18, clone CA1.4E9 further indicate that the antibody clone recognizes an epitope common to a number of mammalian species.
Immunoprecipitation experiments using detergent lysates of iodinated peripheral blood leukocytes indicate that clone Ca1.4E9 immunoprecipitates the common 95 kDa beta 2 integrin chain (CD18) along with the non-covalently associated alpha chains at 180 kDa (CD11a ), 165 kDa (CD11b) and 150 kDa (CD11c). Of note is the lack of precipitation of the integrin alpha D chain (CD11d), this is likely due to the almost complete absence of CD11d expression on peripheral blood lymphocytes, in contrast to expression in the splenic red pulp.
High CD18 expression is a common feature of lymphoma and hyperplasia in dogs.
The tissue and cellular distribution of CD18 in canine tissue closely follows that observed for humans CD18 is expressed by virtually all leucocytes, but more strongly upon monocytes and granulocytes than on lymphocytes. The cross reactivity patterns of mouse anti canine CD18, clone CA1.4E9 further indicate that the antibody clone recognizes an epitope common to a number of mammalian species.
Immunoprecipitation experiments using detergent lysates of iodinated peripheral blood leukocytes indicate that clone Ca1.4E9 immunoprecipitates the common 95 kDa beta 2 integrin chain (CD18) along with the non-covalently associated alpha chains at 180 kDa (CD11a ), 165 kDa (CD11b) and 150 kDa (CD11c). Of note is the lack of precipitation of the integrin alpha D chain (CD11d), this is likely due to the almost complete absence of CD11d expression on peripheral blood lymphocytes, in contrast to expression in the splenic red pulp.
High CD18 expression is a common feature of lymphoma and hyperplasia in dogs.
Product Form:
Purified IgG - liquid.
Preparation:
Purified IgG prepared by affinity chromatography on protein G from ascites.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide (NaN3).
Approx. Protein Concentrations:
IgG concentration 1.0 mg/ml.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry, immunohistology - frozen, immunohistology - paraffin, immunoprecipitation, western blotting, immunofluorescence.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Product Type:
Monoclonal antibody
Clone:
CA1.4E9
Isotype:
IgG1
Specificity:
CD18
Species Cross-Reactivity:
Horse, pig, human, bovine, cat
Target:
Dog
