Mouse anti Dog CD25

Description:

Mouse anti dog CD25, clone P4A10 recognizes the canine homologue of the human CD25 cell surface antigen, also known at IL-2Rα, a glycoprotein of approximately 55 kDa expressed primarily by activated T lymphocytes.
The IL-2 receptor is composed of 3 subunits, an α chain (CD25), a β chain (CD122) and a γ chain (CD132), CD25 functions as a low affinity receptor for IL-2.
Mouse anti human CD25 antibody, clone ACT1, which has demonstrated cross reactivity to canine CD25. However this clone has poor affinity for canine CD25. The development of clone P4A10 offers a specific monoclonal Mouse anti canine CD25, demonstrating a greater affinity for canine CD25 than the cross reactive anti human antibody.

The dog is an important veterinary species in its own right. In addition dogs are used as an animal model in the study of a number of serious human disease states including various forms cancers, and in genetically related diseases of the hemopoietic system.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide.

Carrier Free:

Yes

Immunogen:

Enriched and stimulated Canine T cells.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Fusion Partners:

Spleen cells from immunised RBF/DnJ mice were fused with cells of the mouse P3-653 myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry, Western blotting.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.