Mouse anti Dog CD25:RPE

Description:

Mouse anti dog CD25, clone P4A10 recognizes the canine homologue of the human CD25 cell surface antigen, also known at IL-2Rα, a glycoprotein of approximately 55 kDa expressed primarily by activated T lymphocytes.
The IL-2 receptor is composed of 3 subunits, an α chain (CD25), a β chain (CD122) and a γ chain (CD132), CD25 functions as a low affinity receptor for IL-2.
Mouse anti human CD25 antibody, clone ACT1, which has demonstrated cross reactivity to canine CD25. However this clone has poor affinity for canine CD25. The development of clone P4A10 offers a specific monoclonal Mouse anti canine CD25, demonstrating a greater affinity for canine CD25 than the cross reactive anti human antibody.

The dog is an important veterinary species in its own right. In addition dogs are used as an animal model in the study of a number of serious human disease states including various forms cancers, and in genetically related diseases of the hemopoietic system.

Product Form:

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized.

Reconstitution:

Reconstitute with 1.0 ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial.

Preparation:

Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).
1% Bovine serum albumin.
5% Sucrose.

Immunogen:

Enriched and stimulated Canine T cells.

Fusion Partners:

Spleen cells from immunised RBF/DnJ mice were fused with cells of the mouse P3-653 myeloma cell line.

Excitation Max (nm):

RPE 488 nm laser: 496.

Emission Max (nm):

RPE 488 nm laser: 578.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry: use 10 µl of the suggested working dilution to label 10⁶ cells in 100 µl.

Storage:

Prior to reconstitution store at +4°C.
After reconstitution store at +4°C.

Note:

DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.