Description:
Mouse anti dog CD34 antibody, clone 1H6 recognizes the canine homologue of CD34, a glycosylated type 1 transmembrane protein of approximately 110 kDa expressed on the cell suface of endothelial cells and haematopoietic stem cells.
Mouse anti dog CD34 antibody, clone 1H6 is a key marker of canine hematopoietic progenitor cells and is reported for use in CD34+ enrichment assays.
Product Form:
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized.
Reconstitution:
Reconstitute with 1 ml distilled water.
Preparation:
Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide (NaN3).
1% Bovine serum albumin.
5% Sucrose.
Immunogen:
Canine CD34 fusion protein.
Fusion Partners:
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS-1/FOX-NY myeloma cell line.
Excitation Max (nm):
RPE 488 nm laser: 496.
Emission Max (nm):
RPE 488 nm laser: 578.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
Note:
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Product Type:
Monoclonal antibody