Mouse anti Dog CD4

Description:

Mouse anti dog CD4 antibody (CA13.1E4), a monoclonal antibody specific for canine CD4. CA13.1E4 was clustered at the first Canine Leukocyte Antigen Workshop (Claw) and identifies, by immunoprecipitation a ~60 kDa monomeric protein under both reducing and non-reducing conditions. Canine CD4 is a surface glycoprotein expressed by non CD8 expressing T lymphocytes, with similar developmental and functional characteristics to T helper cells described in other mammalian species. Mouse anti dog CD4 also recognizes a population of CD8 positive cells in the canine thymus.

CD4 expression has also been reported on a population of CD3 positive peripheral blood T lymphocytes which are double positive for CD4 and CD8. Similar reports have been made for cynomolgus monkey, pig as well as rat, chicken and human.

Uniquely amongst mammalian species clone CA13.1E4 also recognizes CD4 expressed on canine neutrophils at a similar density to that expressed on canine T helper cells. The functional significance of this is not understood and remains enigmatic in view of the roles described for CD4 in canine and other mammalian species.

Clone CA13.1E4 also demonstrates immunohistological staining of splenic marginal zone macrophages. Other macrophage populations, such as splenic red pulp macrophages and Langerhans cells do not stain with clone CA13.1E4. Canine CD4 positive T cells can be further characterized according to their simultaneous expression of CD45RA as recognized by rat anti canine anti CD45RA clone CA4.1D3 in a manner analogous to that seen with human T cells.

Clone CA13.1E4 has been used amongst a large panel of anti-canine monoclonal antibodies for the study of various lymphoproliferative diseases. T lymphocytes in large granular lymphocyte (LGL) lymphocytosis are negative for CD4. Dogs with chronic myelogenous leukemia demonstrate CD4 positive staining on neutrophils. CD4 expression varies considerably between various canine leukemias.

Product Form:

Tissue culture supernatant - liquid.

Preservative Stabilisers:

0.1% Sodium azide.

Immunogen:

Canine thymocytes.

Fusion Partners:

Spleen cells from immunised Balb/c mice were fused with cells of the P3X63-Ag.653 mouse myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry, immunohistology - frozen, immunoprecipitation.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.