Mouse anti Duck IgY Heavy Chain

Description:

Mouse anti duck IgY antibody, clone 16C7 recognises both the IgY and IgY(-Fc) Pekin duck heavy chain molecules.

The duck IgY molecule, consisting of two heavy (H) and two light (L) chains, has been categorized as the 7.8S full-length IgY form and the 5.7S truncated IgY(-Fc) form. The IgY(-Fc) molecule possesses the H chain Cu1 and Cu2 constant domains, but is lacking the Cu3 and Cu4 constant region terminal domains, all four of which are present in the full-length IgY molecule. Thus the structure of the IgY(-Fc) molecule resembles that of a F(ab′)₂ fragment of IgY.

Mouse anti duck IgY antibody, clone 16C7 also detects IgY heavy chain in Mallard ducks, goose and swan.

Mouse anti duck IgY antibody, clone 16C7 detects bands of approximately 118 kDa and 178-200 kDa in Pekin duck cell lysates under non-reducing conditions in western blotting.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

< 0.1% Sodium azide (NaN₃).

Immunogen:

Purified Pekin duck yolk IgY.

Approx. Protein Concentrations:

IgG concentration 0.5 mg/ml.

Fusion Partners:

Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

ELISA, flow cytometry, western blotting.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.