Mouse anti Duck IgY Light Chain

Description:

Mouse anti duck IgY light chain antibody, clone 14A3 recognizes the light chain of duck immunoglobulin, expressed strongly on bursal cells and weakly on splenocytes and peripheral lymphoid cells, but not expressed by thymocytes.

Double-staining with clone 14A3 and duck CD8 alpha clone Du CD8-1, reveals the presence of a CD8^high /14A3^- cytotoxic T cell population and a CD8^+/14A3^+ B cell population in duck spleen and also reveals a high percentage of CD8^+/14A3^+ cells in duck bursa. The recognition of B cells by 14A3 using chBAFF-Flag (B cell activating factor).

Mouse anti duck IgY Light Chain antibody, clone 14A3 recognizes IgA in Pekin duck bile and can be used as a marker to identify B cells in both Pekin and Muscovy duck and geese.

Mouse anti duck IgY light chain antibody, clone 14A3 detects a band of approximately 25 kDa in duck cell lysates in Western blotting.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

< 0.1% Sodium azide (NaN₃).

Immunogen:

Purified Pekin duck yolk IgY.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Fusion Partners:

Spleen cells from immunized Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

ELISA, flow cytometry, western blotting.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.