Mouse anti Feline Immunodeficiency Virus p24 gag

Description:

Mouse anti feline immunodeficiency virus p24 gag antibody, clone PAK3-2C1 recognizes an epitope on the C-terminal alpha-helix 9 within the amino-acid sequence AEVKLYLKQSLSIAN of feline immunodeficiency virus (FIV) p24 gag.

The lentivirus FIV, responsible for a progressive and debilitating immune deficiency syndrome in domestic cats, similar to that caused by the human Immunodeficiency Virus (HIV), is a complex retrovirus with a tightly-packed genome, containing the structural genes gag, env and pro-pol and the accessory genes vif, rev and ORF-A/2.

The specific binding epitope recognized by mouse anti feline immunodeficiency virus p24 gag antibody, clone PAK3-2C1 appears to be enclosed in a tight peptide coil during gag production and maturation. The addition of 0.3% Triton-X detergent is recommended to reveal this epitope for antibody binding.

Mouse anti feline immunodeficiency virus p24 gag antibody, clone PAK3-2C1 detects a dominant band of approximately 24kDa, and also detects p24 gag precursor bands at 36, 39, 49 and 52 kDa under reducing conditions in Western blotting.

Mouse anti feline immunodeficiency virus p24 gag antibody, clone PAK3-2C1 does not recognize feline leukaemia virus (FeLV), feline herpes virus (FHV) type 1, Feline coronavirus or feline calicivirus.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

<0.1% Sodium azide (NaN₃).

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

ELISA, flow cytometry, immunofluorescence, immunohistology - frozen, immunohistology - paraffin, western blotting.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.