Mouse anti Guinea Fowl Bu-1a/b

Description:

Mouse anti guinea fowl Bu-1a/b antibody, clone BoA1 recognizes the avian bursal cell antigen, Bu-1a/b. Bu-1a/b, also known as chB6, is a type 1 transmembrane protein.

Bu-1 is a product of two alleles, Bu-1a (chB6.1) and Bu-1b (chB6.2).Mouse anti guinea fowl Bu-1a/b antibody, clone BoA1 recognizes both of these alleles. The Bu-1 antigen is expressed by chicken B-cells throughout most of their development and by a subset of monocytes and macrophages but is absent from erythrocytes, granulocytes and thrombocytes. As such the mouse anti guinea fowl Bu-1a/b antibody, clone BoA1 provides a useful marker of avian B-cells.

Clone BoA1 is unique in that while other antibodies generated against the Bu-1a/b antigen are restricted in their species reactivity, clone BoA1 recognizes a conserved epitope of the Bu-1a/b antigen in both domestic and wild phasianid species

In addition to mouse anti guinea fowl Bu-1a/b antibody, clone BoA1, clone AV20 which is specific to chicken Bu1-a/b and clone L22 which recognizes the Bu-1a but not the Bu-1b allele in chicken and quail, are also available.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.1% Sodium azide (NaN₃).
0.1% Bovine serum albumin.

Immunogen:

Bursal cell suspension from guinea fowl (Numida melagris).

Approx. Protein Concentrations:

IgG concentration 0.5 mg/ml.

Fusion Partners:

Spleen cells from immunized Balb/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Immunohistology - frozen, immunohistology - paraffin, immunoprecipitation,western blotting.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.