Overview

Description:

Mouse anti horse CD11a/CD18 antibody, clone CVS9 recognizes the equine homolog of the human CD11a/CD18 cell surface antigen, a hetrodimer expressed on all equine cells of haemopoietic origin. Equine CD11a/CD18 has higher expression on some subpopulations of T-cells.

In addition to the CVS9 clone, other CVS clones recognising equine MHC and cell surface antigens are available from BioVenic.

Product Form:

Purified IgG conjugated to fluorescein isothiocyanate isomer 1 (FITC) - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN_{31% Bovine serum albumin.}

Immunogen:

Equine leucocytes.

Approx. Protein Concentrations:

IgG concentration 0.1 mg/ml.

Fusion Partners:

Spleen cells from immunised mice were fused with cells of the X63-Ag 8.653 mouse myeloma cell line.

Excitation Max (nm):

FITC: 490.

Emission Max (nm):

FITC: 525.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry: use 10 µl of the suggested working dilution to label 10⁶ cells in 100 µl.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

Product Type:

Monoclonal antibody

Clone:

CVS9

Isotype:

IgG1

Specificity:

CD11a/CD18

Target:

Horse

Mouse anti Horse CD11a/CD18:FITC

Overview

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