Mouse anti Horse CD13:RPE

Description:

Mouse anti horse CD13 antibody, clone CVS19 recognizes the equine CD13 cell surface antigen, also known as Aminopeptidase N, a single-pass type II membrane protein belonging to the peptidase N family with a molecular weight of between 150 - 170 kDa.

CD13 is widely expressed by a range of cell types including all blood neutrophils, basophils, monocytes, fibroblasts, kidney epithelial cells, endothelial cells and mesenchymal stem cells, but not by T or B cells. It is involved in a broad spectrum of biological processes and is believed to be linked to a number of disease states including tumor invasion.

Mouse anti horse CD13 antibody, clone CVS19 may be used for differentiating myeloid and lymphoid linage cells in tumors of the haematopoietic system.

In addition to clone CVS19, other CVS clones recognising equine MHC and cell surface antigens are available.

Product Form:

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized.

Reconstitution:

Reconstitute with 1.0ml distilled water.

Preparation:

Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).
1% Bovine serum albumin.
5% Sucrose.

Immunogen:

Equine leucocytes.

Fusion Partners:

Spleen cells from immunised mice were fused with cells of the mouse X63-Ag8.653 myeloma cell line.

Excitation Max (nm):

RPE 488 nm laser: 496.

Emission Max (nm):

RPE 488 nm laser: 578.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry: use 10 µl of the suggested working dilution to label 10⁶ cells in 100 µl.

Storage:

Store at +4°C.
This product should be stored undiluted. This product is photosensitive and should be protected from light.

Note:

DO NOT FREEZE.